The past decade has been filled with large leaps in terms of technology and how we live. Various new technologies has been integrated into consumer products, but some of the tech that have surfaced these past years aren’t as well known as our household mp3 player or new-age tablet computer, but lies within the chemical industry, studying the way our genetics mould who we are and what we can be.
Polynucleotide research is set to become one of the major branches of biochemistry, and as its popularity is rising, the need for consistency and repeatability in the research processes must be ensured. Various methods can be used to ensure that accurate and precise results are produced. One such way is to use DNA synthesizers that produce a low gene synthesis error rate. This minimizes systematic errors, giving the researcher the closest value to the true of any measurement they may need.
Oglionucleotide synthesis has also experienced significant growth over the last decade as more and more complicated equipment has enabled the production of synthetic DNA and RNA more efficiently. One such produced synthetic oglionucleotide is Poly dA:dT a novel promoter element which is commonly found in yeast promoters. Depending on the length of the structures, it acts as a stimulant for Gcn4 transcription but inversely related to intracellular Gcn4 levels. Poly dA:dT has numerous other uses, but it mainly serves as an extremely effective means of accessing transcription factors that were previously bound to nearby sequences only.
The Midland Certified Reagent Company provides a wide offering of Oglionucleotide synthesis requirements such as Poly dA:dT as well as bespoke DNA and RNA modifications.
In molecular biology experiments, it is important to fabricate Oligonucleotides which is a result of DNA and RNA synthesis. These tiny bits of nucleic acids such as Poly(dA) can be produced in a laboratory through a solid phase chemical synthesis. Oligonucleotides are one of the key ingredients for DNA sequencing, artificial gene synthesis, gene library construction, polymerase chain reaction and as a molecular beacon probe.
Synthetic Oligonucleotide is commonly used to screen viral diseases. It is also used on many molecular biology experiments, most especially on identifying genes or DNA sequencing. In the process of a DNA sequencing, Oligonucleotides are used as primers for the DNA enzymes, it will serve as a pattern for the DNA enzymes to follow. Once synthesized, the DNA enzymes and Oligonucleotides will form a double strand DNA. Another great thing about Oligonucleotides is that it has been a great use for microarrays. These are used to screen genetic diseases such as breast cancer. In recent developments, Oligonucleotide microarrays such as the ViroChip can be used to detect illnesses through a person’s phlegm and other body secretions. This is extremely useful as it has become easier to determine viral infections as symptoms are often the same.
Article submitted by The Midland Certified Reagent Company. The Midland Certified Reagent Company specializes in RNA, LNA, Oligonucleotides, Polynucleotides and DNA synthesis. The company has been a leader in this field for more than 20 years and pioneered in using MALDI-TOF mass spectrometry to ensure that quality is maintained optimum.